Sürdürülebilir Kalkınma Amaçları

• 2

• MakaleAçık Erişim2021Scopus SJRQ2/r/bayburt/13464
  Production, purification, and characterization of Streptomyces sp. strain MPPS2 extracellular pyomelanin pigment

  Bayram, Sinan

  Melanins are natural pigments widely distributed in nature from bacteria to humans. These complex, negatively charged, amorphous, high molecular mass natural biopolymers have many different bioactive properties such as antimicrobial, antiviral, antioxidant, liver protective effects, etc. In this study, some chemical and physical properties of the purified extracellular pyomelanin pigment were investigated via XRD (X-ray diffraction), FT-IR (Fourier transform infrared), and 1H NMR (Nuclear magnetic resonance). Additionally, the melanin pigment producer Streptomyces sp. strain MPPS2 was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and 16S rDNA sequence analysis. 16S rDNA nucleotide sequence analysis result was deposited in NCBI GenBank® under accession number MT825616.

  AtıfScopus28·WoS22·OpenAlex27

  İncele
• MakaleAçık Erişim2020Scopus SJRQ2/r/bayburt/13256
  Bioproduction, structure elucidation and in vitro antiproliferative effect of eumelanin pigment from Streptomyces parvus BSB49

  Bayram, Sinan; Dengiz, Cagatay; Gerçek, Yusuf Can; Cetin, Idil; Topcul, Mehmet R.

  In this study, the structure of the purified extracellular eumelanin pigment isolated from Streptomyces spp. was elucidated by detailed analysis via two different spectroscopic techniques (FT-IR and NMR). In vitro antiproliferative effects of eumelanin were evaluated on HeLa cell line. These experiments were carried out with the evaluation of the parameters including cell viability, cell index, and mitotic index. With the cell viability and cell index, IC50 concentration of eumelanin was determined as 10 μM. This result showed that the IC50 concentration of eumelanin decreased the values of cell viability, cell index and mitotic index. These changes are statistically significant (p ' 0.01). The ability of the dissolved eumelanin (250 μg mL−1) to scavenge free radicals was determined via DPPH and ABTS and was shown to be about 87.73% and 75.2%, respectively, compared with standard antioxidants. It was observed that dry weights of eumelanin yield among the selected strains ranged from 160 to 240 mg L−1. The strain with the highest production potential was selected for 16S rDNA sequence analysis and, accordingly, the selected strain BSB49 was identified as Streptomyces parvus and the sequence analysis results were deposited in NCBI under accession number MK894155.

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  AtıfScopus47·WoS40·OpenAlex48

  İncele
• MakaleAçık Erişim2015Scopus SJRQ2/r/bayburt/13742
  Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains

  Mercan, Emin; İspirli, Hümeyra; Sert, Durmuş; Yılmaz, Mustafa Tahsin; Dertli, Enes

  The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces. Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4. Analysis of EPS production levels showed significant alterations among strains tested. Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production. Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level. An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels. This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.

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  AtıfScopus19·WoS16·OpenAlex21

  İncele