Sürdürülebilir Kalkınma Amaçları

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• MakaleYalnız künye2025Scopus SJRQ2/r/bayburt/11806
  A novel exopolysaccharide from cold-adapted yeast Rhodotorula glutinis, along with structural, rheological, antioxidant, and antibiofilm properties

  Oztekin, Sebahat; Dikmetas, Dilara Nur; Karbancıoglu-Guler, Funda

  In the present study, a novel exopolysaccharide EPS-BMD26 produced by cold-adapted yeast Rhodotorula glutinis was structurally examined, along with its rheological, antioxidant, and antibiofilm properties. High-performance liquid chromatography (HPLC) analysis revealed glucose and galactose as two monosaccharide constituents. The functional groups within the EPS-BMD26 structure were detected by Fourier transform infrared (FT-IR) spectroscopy. The 1H and 13C nuclear magnetic resonance (NMR) spectra of EPS-BMD26 also confirmed its β-D-glucan type with galactose residues with an average molecular weight of 118 kDa. Differential scanning calorimetry (DSC) analysis revealed that EPS-BMD26 had a high level of thermal stability, up to 326.16 °C. Scanning electron microscopy (SEM) analysis demonstrated that EPS-BMD26 had a porous microstructure with fissures. X-ray diffraction (XRD) analysis revealed its semi-crystalline nature. EPS-BMD26 showed moderate and concentration-dependent antioxidant potential recruiting different methods, including •OH, DPPH•, CUPRAC, and ABTS radical scavenging activities. Water-soluble EPS-BMD26 exhibited high water holding capacity of 190 ± 0.22% with a water solubility index of 60.6 ± 8.3%. At 1.25 mg mL-1, EPS-BMD26 inhibited biofilm formation of Staphylococcus aureus ATCC 25923 by 79.5%. Rheological characterization revealed that the aqueous solution of EPS-BMD26 had shear thinning and pseudoplastic behavior. Given these properties, EPS-BMD26 may be a promising bio-hydrocolloid, antibiofilm agent, and food additive for industrial applications.

  AtıfScopus15·WoS15·OpenAlex17

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• MakaleYalnız künye2024Scopus SJRQ2/r/bayburt/13055
  Effect of different carbon sources on exopolysaccharide production of Lactiplantibacillus pentosus KS27 and optimization of production conditions using Box-Behnken designing

  Bekar, Fevzi; Kamiloglu, Aybike

  This study aimed to determine the exopolysaccharide (EPS) production of Lactiplantibacillus pentosus KS27 depending on temperature, incubation time, and medium composition and to determine the optimum conditions. Characterization was carried out for the exopolysaccharide produced under optimum conditions. In the study, three different conditions (25 °C, 35 °C, 45 °C) were used for temperature, glycerol, and sucrose as media, and three different periods (24 h, 36 h, and 48 h) were used. It was determined that the solubility of EPS produced under optimal conditions had the highest water content (98 ± 0.01%), while the water binding capacity was also found to be as high as 1970 ± 0.01% and complied with common EPS characteristics. Differential scanning calorimeter analysis showed a gradual deterioration up to 191.88 °C and a slow decline up to 400 °C. FTIR analysis deciphered peak values between 3289 and 517 cm−1, and functional groups were detected. When the nuclear magnetic resonance (NMR) spectrum was examined, a heterogeneous structure appropriate to the structure of EPS was found by defining the peak entities in three separate regions.

  AtıfScopus5·WoS5·OpenAlex6

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• MakaleAçık Erişim2023Scopus SJRQ2/r/bayburt/12841
  Efficient production of acetone butanol ethanol from sole fresh and rotten potatoes by various Clostridium strains

  Avcı, Ayşe; Kamiloğlu, Aybike; Dönmez, Sedat

  This study aimed to evaluate rotten potato in the fermentation of acetone butanol ethanol (ABE). For this purpose, three strains, Clostridium acetobutylicum DSMZ 1731, Clostridium acetobutylicum DSMZ 6228, and Clostridium beijerinckii DSMZ 6422, were used and production experiments were performed in a simple media only composed of either fresh or rotten potato. Effects of potato concentration (100–400 g/L) and time (24–144 h) on the fermentation were determined using fresh potato. The highest ABE production was detected at 400 g/L potato concentration at the end of 96 h by all strains. Clostridium acetobutylicum DSMZ 6228 was the most efficient strain in producing ABE among the microorganisms tested. Total ABE productions by Clostridium acetobutylicum DSMZ 1731, Clostridium acetobutylicum DSMZ 6228, and Clostridium beijerinckii DSMZ 6422 at 400 g/L in 96 h were 12.3, 17.6, and 12.0 g/L, respectively. The majority of the products was butanol that made up ca. 66–67% of total ABE. Significant amounts of ABE were also produced with rotten potato which was compatible with the results obtained with fresh potato. They were determined as 11.5, 17.1, and 9.6 g/L with Clostridium acetobutylicum DSMZ 1731, Clostridium acetobutylicum DSMZ 6228, and Clostridium beijerinckii DSMZ 6422, respectively. The results showed that rotten potato could be a good candidate as substrate for ABE fermentation.

  AtıfScopus18·WoS16

  İncele
• MakaleYalnız künye2023Scopus SJRQ2/r/bayburt/12839
  Optimization of microwave-assisted biosynthesis of silver nanoparticle with tarragon extract

  Altuncu, Fatma Kübra; Kamiloğlu, Aybike

  Response surface method was used to optimize microwave-assisted silver nanoparticle production with tarragon extract. As a result of the optimization study, tarragon silver nanoparticles (TAgNPs) were produced from 43 conditions with a degree of desirability of 1, with a processing time of 5.25 min, AgNO3 concentration of 9.89 mM, and tarragon extract concentration of 14.80%. Among the variable parameters, AgNO3 concentration affected TAgNP synthesis depending on processing time and tarragon extract concentration; tarragon extract concentration was found to significantly affect nanoparticle formation. TAgNP produced under optimum conditions was found to be crystalline, with a spherical surface morphology and an average particle size of 16.56 nm, with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction diffractometry (XRD) analyses. TAgNPs showed inhibitory effect for Escherichia coli BC 1402, Bacillus cereus BC 6830, Salmonella typhimurium RSSK 95091, Yersinia enterocolitica ATCC 27729, and Staphylococcus aureus ATCC 25923 microorganisms. The minimum inhibition concentrations were 10 µg/mL for B. cereus BC 6830, 50 µg/mL for E. coli BC 1402, S. typhimurium RSSK 95091, and Y. enterocolitica ATCC 27729, and 25 µg/mL for S. aureus ATCC 25923.

  AtıfScopus2·WoS2·OpenAlex2

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• MakaleYalnız künye2022Scopus SJRQ3/r/bayburt/12878
  Optimization of Ultrasound Extraction of Phenolic Compounds from Tarragon (Artemisia dracunculus L.) Using Box–Behnken Design

  Kutlu, Naciye; Kamiloglu, Aybike; Elbir, Tugba

  In this study, ultrasound extraction of bioactive compounds from tarragon was examined as a sustainable technology. Pure water, which is a green solvent, was used as solvent. Box–Behnken was used in the experimental design, and ultrasonic power, extraction time and solvent-to-sample ratio were determined to be independent variables. The physicochemical properties were determined by physical (color, pH, Brix) and chemical analyses (antioxidant activity, total flavonoid and total phenolic compound (TPC)) in the extracts obtained under different conditions. Optimum conditions were found to be 73.9% ultrasonic power, 43.2 min sonication time and 10 mL/g solvent-to-sample ratio. The composite desirability (R2) of optimum conditions was 99.3% after optimization. Since high efficiency was obtained in a short time with ultrasound extraction, it was found to be quite good in terms of energy savings. That is, the highest TPC was obtained with a sonication time of 45 min with this method, while similar TPC in the control group (maceration) was obtained in 24 h. In the optimum conditions, extraction with other green solvents (ethanol and deep eutectic solvent—DES) was carried out and bioactive compound yields were compared. For different solvents compared under optimum conditions, ethanol gave higher yields in terms of TPC, while higher yields were obtained in samples using water as solvent for some antioxidant activity values. The results obtained with DES were not as high as expected, and this resulted in the conclusion that a hydrogen bond donor is not suitable for obtaining bioactive compounds from tarragon.

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  AtıfScopus9·WoS9·OpenAlex9

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