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- MakaleYalnız künye2025Scopus SJRQ2A novel exopolysaccharide from cold-adapted yeast Rhodotorula glutinis, along with structural, rheological, antioxidant, and antibiofilm properties
Oztekin, Sebahat; Dikmetas, Dilara Nur; Karbancıoglu-Guler, Funda
In the present study, a novel exopolysaccharide EPS-BMD26 produced by cold-adapted yeast Rhodotorula glutinis was structurally examined, along with its rheological, antioxidant, and antibiofilm properties. High-performance liquid chromatography (HPLC) analysis revealed glucose and galactose as two monosaccharide constituents. The functional groups within the EPS-BMD26 structure were detected by Fourier transform infrared (FT-IR) spectroscopy. The 1H and 13C nuclear magnetic resonance (NMR) spectra of EPS-BMD26 also confirmed its β-D-glucan type with galactose residues with an average molecular weight of 118 kDa. Differential scanning calorimetry (DSC) analysis revealed that EPS-BMD26 had a high level of thermal stability, up to 326.16 °C. Scanning electron microscopy (SEM) analysis demonstrated that EPS-BMD26 had a porous microstructure with fissures. X-ray diffraction (XRD) analysis revealed its semi-crystalline nature. EPS-BMD26 showed moderate and concentration-dependent antioxidant potential recruiting different methods, including •OH, DPPH•, CUPRAC, and ABTS radical scavenging activities. Water-soluble EPS-BMD26 exhibited high water holding capacity of 190 ± 0.22% with a water solubility index of 60.6 ± 8.3%. At 1.25 mg mL-1, EPS-BMD26 inhibited biofilm formation of Staphylococcus aureus ATCC 25923 by 79.5%. Rheological characterization revealed that the aqueous solution of EPS-BMD26 had shear thinning and pseudoplastic behavior. Given these properties, EPS-BMD26 may be a promising bio-hydrocolloid, antibiofilm agent, and food additive for industrial applications.
AtıfScopus15WoS15OpenAlex17İncele
- BildiriYalnız künye2013Nonlinear analysis of structures by Total Potential Optimization using Metaheuristic Algorithms (TPO/MA)
Toklu, Y.C.; Bekdaş, G.; Temür, R.; Toklu, Ş.Ç.; Uzun, F.; de Wilde, P.; Suter, G.; Rafiq, Y.
Structural analysis problems can be formulized as either root finding problems, or optimization problems. The general practice is to choose the first option directly or to convert the second option again to a root finding problem by taking relevant derivatives and equating them to zero. The second alternative is used very randomly as it is and that only for some simple demonstrative problems, most probably due to difficulty in solving optimization problems by classical methods. The method called Total Potential Optimization using Metaheuristic Algorithms (TPO/MA) described in this study successfully enables handling structural problems with optimization formulation. Using metaheuristic algorithms provides additional advantages in dealing with all kinds of constraints.
AtıfScopus1İncele
