2026-10-29T22:40:33Zhttps://bayburt-rdlopen.rawdatalibrary.net/oai/request

oai:bayburt-rdlopen.rawdatalibrary.net:bayburt/145082026-10-29T19:44:11Zopenairedrivercom_bayburt_10000col_bayburt_10001

Investigation of the effect of gossypin on MMP-2 and MMP- 9 genes in prostate cancer cellsDirican, EbubekirÇinar, IrfanGossypinMMP-2MMP-9PC-3RT-PCRPurpose: The aim of this study is to explore the effects of gossypin on matrix metalloproteinases -2 (MMP-2) and MMP-9 genes in prostate cancer cells. Materials and Methods: PC3 cells were grown in culture and treated with three different concentrations of gossypin (25-50-100 mu g/ml) and cisplatin (50 mu M) as a positive control. First, RNA isolation was performed. Then, cDNA synthesis was performed and RT-PCR was used to evaluate mRNA expression levels of MMP-2 and MMP-9 genes. Results: Gossypin decreased MMP-2 and MMP-9 mRNA expression in prostate cancer cells in a concentrationdependent manner. Three concentrations (25-50-100 mu g/ml) of gossypin in PC3 cells reduced the mRNA expression of the MMP-2 gene. While the fold change value of MMP-2 gene expression was 0.3482 +/- 0.040 in the 100 mu g/ml gossypin group, it was 1.007 +/- 0.1425 in the control group. In addition, 50 mu g/ml and 100 mu g/ml concentrations of gossypin decreased the mRNA expression of the MMP-9 gene. The expression level of the MMP-9 gene in prostate cancer cells was 0.4740 +/- 0.038 in the 100 mu g/ml gossypin administered group, while it was 1.009 +/- 0.1687 in the control group. There was a positive correlation between the expressions of the MMP-2 and MMP-9 genes. Conclusion: According to the results obtained, it is seen that gossypin reduces the expression of MMP-2 and MMP9 genes in prostate cancer cells and the effects of gossypin on other genetic and epigenetic mechanisms in cancer need to be investigated to reveal the anti-cancer.CUKUROVA UNIV, FAC MEDICINE2022Articlehttps://rdlopen.rawdatalibrary.net/r/bayburt/14508https://doi.org/10.17826/cumj.11373062602-3032enCUKUROVA MEDICAL JOURNALinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://bayburt-rdlopen.rawdatalibrary.net/tr/items/bayburt/14508