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oai:bayburt-rdlopen.rawdatalibrary.net:bayburt/141352026-10-29T20:01:39Zopenairedrivercom_bayburt_10000col_bayburt_10001com_bayburt_10007col_bayburt_10227

The Phenolic Profile and Anti-Inflammatory Effect of Ethanolic Extract of Polish Propolis on Activated Human Gingival Fibroblasts-1 Cell LineKurek-Górecka, AnnaKlósek, MalgorzataPietsz, GrazynaCzuba, Zenon P.Kolayli, SevgiCan, ZehraBalwierz, RadoslawOlczyk, Pawelcaffeic acid phenethyl estercytokinesinflammationin vitro modelpolyphenolspropolisPropolis, owing to its antibacterial and anti-inflammatory properties, acts as a cariostatic agent, capable of preventing the accumulation of dental plaque and inhibiting inflammation. The anti-inflammatory properties of propolis are attributed to caffeic acid phenethyl ester (CAPE), which is present in European propolis. The objective of the conducted study was to assess the anti-inflammatory effects of the Polish ethanolic extract of propolis (EEP) and isolated CAPE on stimulated with LPS and IFN-alpha, as well as the combination of LPS and IFN-alpha. The cytotoxicity of the tested compounds was determined using the MTT assay. The concentrations of specific cytokines released by the HGF-1 cell line following treatment with EEP (25-50 mu g/mL) or CAPE (25-50 mu g/mL) were assessed in the culture supernatant. In the tested concentrations, both CAPE and EEP did not exert cytotoxic effects. Our results demonstrate that CAPE reduces TNF-alpha and IL-6 in contrast to EEP. Propolis seems effective in stimulating HGF-1 to release IL-6 and IL-8. A statistically significant difference was observed for IL-8 in HGF-1 stimulated by LPS+IFN-alpha and treated EEP at a concentration of 50 mu g/mL (p = 0.021201). Moreover, we observed that CAPE demonstrates a stronger interaction with IL-8 compared to EEP, especially when CAPE was administered at a concentration of 50 mu g/mL after LPS + IFN-alpha stimulation (p = 0.0005). Analysis of the phenolic profile performed by high-performance liquid chromatography allowed identification and quantification in the EEP sample of six phenolic acids, five flavonoids, and one aromatic ester-CAPE. Propolis and its compound-CAPE-exhibit immunomodulatory properties that influence the inflammatory process. Further studies may contribute to explaining the immunomodulatory action of EEP and CAPE and bring comprehensive conclusions.MDPI2023-11Articlehttps://rdlopen.rawdatalibrary.net/r/bayburt/14135https://doi.org/10.3390/molecules28227477enMOLECULESinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://bayburt-rdlopen.rawdatalibrary.net/tr/items/bayburt/14135