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oai:bayburt-rdlopen.rawdatalibrary.net:bayburt/128742026-10-29T19:42:51Zopenairedrivercom_bayburt_10000col_bayburt_10001com_bayburt_10010col_bayburt_10222

Interleukin-27 as a novel player in alleviating hepatic steatosis: Mechanistic insights from an in vitro analysisCho, WonjunOh, HeeseungAbd El-Aty, A.M.Özten, ÖmerJeong, Ji HoonJung, Tae WooAMPKAutophagyER stressIL-27NAFLDObesityInterleukin-27 (IL-27) is a recently discovered cytokine that has been implicated in inflammatory and metabolic conditions, such as atherosclerosis and insulin resistance. However, the mechanisms by which IL-27 attenuates hepatic lipid accumulation in hyperlipidemic conditions and counteracts endoplasmic reticulum (ER) stress, a known risk factor for impaired hepatic lipid metabolism, have not been elucidated. This in vitro study was designed to examine the effect of IL-27 on hepatic lipid metabolism. The study included the evaluation of lipogenesis-associated proteins and ER stress markers by Western blotting, the determination of hepatic lipid accumulation by Oil Red O staining, and the examination of autophagosome formation by MDC staining. The results showed that IL-27 treatment reduced lipogenic lipid deposition and the expression of ER stress markers in cultured hepatocytes exposed to palmitate. Moreover, treatment with IL-27 suppressed CD36 expression and enhanced fatty acid oxidation in palmitate-treated hepatocytes. The effects of IL-27 on hyperlipidemic hepatocytes were attenuated when adenosine monophosphate-activated protein kinase (AMPK) or 3-methyladenine (3 MA) were inhibited by small interfering RNA (siRNA). These results suggest that IL-27 attenuates hepatic ER stress and fatty acid uptake and stimulates fatty acid oxidation via AMPK/autophagy signaling, thereby alleviating hepatic steatosis. In conclusion, this study identified IL-27 as a promising therapeutic target for nonalcoholic fatty liver disease (NAFLD).Elsevier B.V.2024Articlehttps://rdlopen.rawdatalibrary.net/r/bayburt/12874https://doi.org/10.1016/j.bbrc.2024.1496710006-291XenBiochemical and Biophysical Research Communicationsinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://bayburt-rdlopen.rawdatalibrary.net/tr/items/bayburt/12874