2026-10-29T22:40:19Zhttps://bayburt-rdlopen.rawdatalibrary.net/oai/request

oai:bayburt-rdlopen.rawdatalibrary.net:bayburt/127322026-10-29T19:42:44Zopenairedrivercom_bayburt_10000col_bayburt_10001com_bayburt_10007col_bayburt_10225

Effects of graviola oil extract on fattening performance and circadian rhythms of adipokine, cardiac and mitochondrial function markers in lambsKotan, Gökşad CemilBayraktar, BülentApelinBrain and muscle ARNT-like protein 1 (BMAL1) and mitochondrial functionBrain Mitochondrial Carrier Protein-1 (BMCP1)Cardiac troponin I (cTnI)Circadian RhythmGraviolaLambMitochondrial-derived peptide (MOTS-c)Background: This study investigated the effects of dietary graviola (Annona muricata L.) oil extract (GOE) (supplemented at 200, 400, and 600 mg/kg) on the growth performance and circadian rhythm profiles of adipokine, cardiac, and mitochondrial function biomarkers in 48 male Anatolian Merino lambs (2.5 months old). Methods: Lambs were assigned to a control group (G0) or supplementation groups (G200, G400, G600 mg/kg) for a 60-day trial (70 days total). The study was conducted under a 12-h light/12-h dark cycle to ensure controlled environmental conditions. To analyze circadian profiles, blood samples were collected on days 0, 15, 30, 45, and 60 at four time points (07:00, 13:00, 19:00, and 01:00). Serum levels of Apelin, cTnI, BMAL1, MOTS-c, and BMCP1 were measured by ELISA. Data were analyzed using GLM Multivariate analysis (p < 0.05). Results: Key findings showed the G400 dose resulted in the most effective linear increase in live weight and live weight gain by day 60, and had a significant overall effect on live weight (p < 0.05). The G200 dose significantly improved the feed conversion ratio (p < 0.05), while feed consumption was unaffected (p > 0.05). Regarding biomarkers on day 60, the G400 dose effectively modulated BMAL1, MOTS-c, and BMCP1 (peak at 19:00). The G600 dose yielded the best results for Apelin (peak at 19:00) and cTnI (peaks at 13:00 and 07:00). Conclusions: In conclusion, GOE at a dose of 400 mg/kg (G400) not only optimizes fattening performance in Anatolian Merino lambs but also supports metabolic homeostasis by modulating adipokine levels, cardiac function, and mitochondrial biogenesis processes in a manner consistent with the circadian rhythm. These findings demonstrate that the G400 dose is a safe and effective phytogenic strategy for improving animal health and production efficiency.BioMed Central Ltd2026Articlehttps://rdlopen.rawdatalibrary.net/r/bayburt/12732https://doi.org/10.1186/s12917-026-05400-31746-6148enBMC Veterinary Researchinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://bayburt-rdlopen.rawdatalibrary.net/tr/items/bayburt/12732