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oai:bayburt-rdlopen.rawdatalibrary.net:bayburt/119952026-10-29T19:42:04Zopenairedrivercom_bayburt_10000col_bayburt_10001com_bayburt_10007col_bayburt_10223

Bifidogenic effect and in vitro immunomodulatory roles of melibiose-derived oligosaccharides produced by the acceptor reaction of glucansucrase E81İspirli, HümeyraKaya, YaseminDertli, EnesFunctional rolesGlucansucraseImmune-ModulationMelibiose-Derived oligosaccharidesPrebioticsGlucansucrases are responsible for the production of α-glucans using sucrose as the substrate. Glucansucrases may also produce different oligosaccharides by transferring the glucose moiety from sucrose to a variety carbohydrates acting as acceptor nucleophile. In this study, melibiose-derived oligosaccharides were produced by the glucansucrase from Lactobacillus reuteri E81 expressed without the N-terminal region (GtfA-ΔN). The reaction products were characterized by TLC, LC-MS and NMR analysis and it was found that GtfA-ΔN synthesized melibiose-derived oligosaccharides (DP3 and DP4) by adding glucose units through alpha 1->3 or 1->6 glycosidic bond. The functional characteristics of these melibiose-derived oligosaccharides were determined by testing the immune-modulatory functions in HT-29 cells and testing their growth promoting effects for important probiotic and pathogenic strains. The melibiose-derived oligosaccharides triggered the production of anti-inflammatory cytokine IL-10 and pro-inflammatory cytokine TNF-α depending on their concentrations. Finally, melibiose-derived oligosaccharides showed bifidogenic effect as potential prebiotics.Elsevier Ltd2020Articlehttps://rdlopen.rawdatalibrary.net/r/bayburt/11995https://doi.org/10.1016/j.procbio.2019.12.0031359-5113enProcess Biochemistryinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://bayburt-rdlopen.rawdatalibrary.net/tr/items/bayburt/11995