2026-10-30T00:49:28Zhttps://bayburt-rdlopen.rawdatalibrary.net/oai/requestoai:bayburt-rdlopen.rawdatalibrary.net:bayburt/118062026-10-29T19:41:54Zopenairecom_bayburt_10000col_bayburt_10001com_bayburt_10007col_bayburt_10223A novel exopolysaccharide from cold-adapted yeast Rhodotorula glutinis, along with structural, rheological, antioxidant, and antibiofilm propertiesOztekin, SebahatDikmetas, Dilara NurKarbancıoglu-Guler, FundaAntibiofilmAntioxidantExopolysaccharideRheologyRhodotorula glutinisStructural analysisIn the present study, a novel exopolysaccharide EPS-BMD26 produced by cold-adapted yeast Rhodotorula glutinis was structurally examined, along with its rheological, antioxidant, and antibiofilm properties. High-performance liquid chromatography (HPLC) analysis revealed glucose and galactose as two monosaccharide constituents. The functional groups within the EPS-BMD26 structure were detected by Fourier transform infrared (FT-IR) spectroscopy. The 1H and 13C nuclear magnetic resonance (NMR) spectra of EPS-BMD26 also confirmed its β-D-glucan type with galactose residues with an average molecular weight of 118 kDa. Differential scanning calorimetry (DSC) analysis revealed that EPS-BMD26 had a high level of thermal stability, up to 326.16 °C. Scanning electron microscopy (SEM) analysis demonstrated that EPS-BMD26 had a porous microstructure with fissures. X-ray diffraction (XRD) analysis revealed its semi-crystalline nature. EPS-BMD26 showed moderate and concentration-dependent antioxidant potential recruiting different methods, including •OH, DPPH•, CUPRAC, and ABTS radical scavenging activities. Water-soluble EPS-BMD26 exhibited high water holding capacity of 190 ± 0.22% with a water solubility index of 60.6 ± 8.3%. At 1.25 mg mL-1, EPS-BMD26 inhibited biofilm formation of Staphylococcus aureus ATCC 25923 by 79.5%. Rheological characterization revealed that the aqueous solution of EPS-BMD26 had shear thinning and pseudoplastic behavior. Given these properties, EPS-BMD26 may be a promising bio-hydrocolloid, antibiofilm agent, and food additive for industrial applications.Springer Science and Business Media Deutschland GmbH2025Articlehttps://rdlopen.rawdatalibrary.net/r/bayburt/11806https://doi.org/10.1007/s13399-023-05208-32190-6815enBiomass Conversion and Biorefineryinfo:eu-repo/semantics/closedAccessinfo:eu-repo/semantics/articlehttps://bayburt-rdlopen.rawdatalibrary.net/tr/items/bayburt/11806